Friday, March 1, 2024
HomeDr. Debabrata Mandal

People

Quick Links

Notification Center

Dr. Debabrata Mandal

Associate Professor

Dept. of Biotechnology

dmandal@niperhajipur.ac.in;debabrataman@gmail.com

+91-8335996348

Date of joining as the Assistant Professor (contractual) – 14.08.2014

Date of Joining as Associate Professor (regular) – 06.10.2020

Faculty CV

Academics
2007: Ph.D (Biochemistry, Molecular Biology) from Bose Institute, Kolkata (Jadavpur University) (Title of PhD thesis – Insights into the mechanisms governing maintenance of structure and function of human erythrocyte membrane)

1998: M.Sc. in Biotechnology from IIT-Mumbai

1996: B.Sc. in Chemistry (Hons.), (Physics and Mathematics as additional subjects) from Ramkrishna Mission Residential College, Narendrapur (University of Calcutta)

Research Areas

  • Nano-biotechnology and Nano-medicine
  • Antimicrobial efficacy of green synthesized biomaterials
  • Biosensor with functionalized gold nanoparticles
  • Biochemistry and Molecular Biology
  • Quantam-dot based drug delivery for antimicrobial resistance

Broad research Area My research area involves drug delivery against neglected tropical diseases like leishmaniasis using nanoparticles. Further, drug delivery against fungal and bacterial infections using quantam dot are also explored. Delivery of drugs followed by identification of mechanism of action using biochemical, molecular biology and proteomics approach is the area of our expertise. Generation of recombinant proteins, enzyme assay and drug binding interactions with ITC and CD are done to quantify the drug-target binding interactions. In our recent work, we have identified natural compounds like medium chain fatty acids (caprylic acid), flavonoids (7, 8-dihydroxyflavone) etc. as key antiparasitic agents. The ergosterol biosynthesis and polyamine biosynthesis pathway of parasite was found to be the key target for these antileishmanial agents. We have developed an AmB-based silver NP (AmB-AgNP) which is highly selective in biding with ergosterol but not with cholesterol. The AmB-AgNP has good antileishmanial and anti fungal efficacy. In another work, we are exploring antibiotic synthesized quantam dots as antimicrobials for multi-drug resistant bacteria with possible mechanism of action. We have recently checked the specific nanozyme activity of a QD synthesized from coal waste. The kinetics, ITC and biological studies have proven that the QD is highly specific for superoxide dismutase activity. We are trying to develop a polypeptide vaccine composed of fused peptides of Leishmania origin. The high immunogenicity and TLR binding of the vaccine construct was confirmed by immunoinformatics studies.

Total Published Papers – 35

Total Impact factor = 207

H index = 12

Total Patents = 2

Total PhD degree awarded – 3

PhD submitted – 1

Distinctions

  • Invited Speaker at “World Congress in Biotechnology, New Delhi – 2015”
  • Invited speaker at PSI-IICB international conference during November, 2022
  • Post-doctoral research fellowship at MIT, of Biology –USA from January, 2006 till July, 2012.
  • Awarded “Nilratan Sirkar Prize -2001” from Bose Institute during D.

Experience

  • Presently working as Associate Professor in of Biotechnology, NIPER- Hajipur from 27st August, 2018.
  • Worked as Assistant Professor in of Biotechnology, NIPER- Hajipur from August, 2014 till August, 2018.
  • Worked as Scientist –II at Vyome Biosciences Private limited, Delhi from August, 2012 till July, (The domain of Research – Dermatology and fungal Biology)
  • Research Scientist at Massachusetts Institute of Technology (MIT), Dept. of Biology –USA from January, 2006 till July, 2012 (The research domain – Role of novel tRNA modifications in regulation of protein synthesis

Selected Research Publications

  1. Kumar, P., Kumar, P., Shrivastava, A., Dar, MA., Lokhande, KB., Singh, N., Singh, A., Velayutham, R. and Mandal, D. Immunoinformatics-based multi-epitope containing fused polypeptide vaccine design against visceral leishmaniasis with high immunogenicity and TLR binding. Int J Biol Macromol. 2023 Oct 20;253(Pt 8):127567. doi: 10.1016/j.ijbiomac.2023.127567. Online ahead of print. (IF= 8.2)
  2. Kumbhar, P., Kumar, P., Lasure, A., Velayutham, R. and Mandal, D. An updated landscape on nanotechnology-based drug delivery, immunotherapy, vaccinations, imaging, and biomarker detections for cancers – recent trends and future directions with clinical success. Discover Nano (accepted) 2023. Doi: 10.1186/s11671-023-03913-6.(IF= 5.4)
  3. Das, T., Das, S., Kumar, P., Betty, C., and Mandal, D. Coal waste-derived synthesis of yellow oxidized graphene quantum dots with highly specific superoxide dismutase activity: characterization, kinetics, and biological studies. Nanoscale. 2023 Oct 27. doi: 10.1039/d3nr04259f. Online ahead of print. (IF= 6.6)
  4. Naskar, S., Kowalczyk, D., Mal, S., Das, S., Mandal, D., Kumar, P. and Dirk Ziegenbalg, D. Making photochemistry scalable – an operationally simple falling film looping photoreactor. Chem. Eng., 2023, 8, 2211-2222. DOI: 10.1039/D3RE00107E. (IF= 4.9)
  5. Prasad, S, Kumar, P., Mandal, S.,Mohan, A., …Ravichandiran, V., Das, P., Mandal, D. Mechanistic insight into the role of enzyme mevalonate kinase by a natural fatty acid-mediated killing of Leishmania donovani. (2022). Scientific Reports. Sep 30;12(1):16453. doi: 10.1038/s41598-022-20509-9. (IF = 5.0)
  6. Hassan, A., Saritha, C., Rajana, V., Mandal, D., Das, N. Rationally Designed Ionic Covalent Organic Networks (iCONs) with Efficient Antimicrobial Activities. ACS Macro Letters. 2023 Mar 21;12(3):376-381. doi: 10.1021/acsmacrolett.2c00686. Epub 2023 Feb 2. (IF = 6.1)
  7. Kumar, P., Kumar, P., …. Mandal, D., Velayutham, R. Limitations of current chemotherapy and future of nanoformulation-based AmB delivery for visceral leishmaniasis-An updated review. Frontiers in Bioengineering and Biotechnology. 2022. Dec 14;10:1016925. doi: 10.3389/fbioe.2022.1016925. eCollection 2022. (IF = 5.8)
  8. Dubey, AK., Kumar, P., Mandal, D., Ravichandiran, V., Singh, SK. An introduction to dynamic nucleoporins in Leishmania species: Novel targets for tropical-therapeutics. Journal of Parasitic Diseases 2022 Dec;46(4):1176-1191. doi: 10.1007/s12639-022-01515-0
  9. Kumar, P., Kumar, Pawan., Mandal, D. and Ravichandiran, V. The emerging role of Deubiquitinases (DUBs) in parasites: A foresight review. Frontiers in Cellular and Infection Microbiology. 2022 Sep 27;12:985178. doi: 10.3389/fcimb.2022.985178 (IF= 6.0)
  10. Nayak,S., Kumar, P., Ravi Shankar, Mukhopadhyay, AK., Mandal, D., Das, P. Biomass derived self-assembled DNA-dot hydrogels for enhanced bacterial annihilation. Nanoscale. 2022 Oct 13. doi: 10.1039/d2nr03810b (IF = 6.6)
  11. Prasanna, P., Kumar, P., Mandal, S., Payal, T., Kumar, S., Hossain, U., Das, P., Ravichandiran, V. and Mandal, D. 7,8-dihydroxyflavone-functionalized gold nanoparticles target the arginase enzyme of Leishmania donovani. Nanomedicine (Lond). 2021 Sep;16(21):1887-1903. doi: 10.2217/nnm-2021-0161. (IF = 6.0)
  12. Prasanna, P., Kumar, P., Kumar, S., Rajana, VK., Vishnu Kant., Prasad SR, Mohan, U., Ravichandiran, V. and Mandal, D. Current status of nanoscale drug delivery and the future of nano-vaccine development for leishmaniasis – A review. Biomedicine and Pharmacotherapy 2021 Sep;141:111920. doi: 10.1016/j.biopha.2021.111920. Epub 2021 Jul 20. (IF = 7.4)
  13. Kant, V., Kumar, P., Ranjan, R., Kumar, P., Mandal, D., Vijayakumar. S. In silico screening, molecular dynamic simulations, and in vitro activity of selected natural compounds as an inhibitor of Leishmania donovani 3-mercaptopyruvate sulfurtransferase. Parasitol Res. 2022 Jul;121(7):2093-2109. doi: 10.1007/s00436-022-07532-5. Epub 2022 May 10 (IF = 2.3)
  14. Nayak, S., Prasad, SR., Mandal, D. and Das, P. Hybrid DNA-Carbon Dot-Poly(vinylpyrrolidone) Hydrogel with Self-Healing and Shape Memory Properties for Simultaneous Trackable Drug Delivery and Visible-Light-Induced Antimicrobial Photodynamic Inactivation. ACS Appl Bio Mater. 2020 Nov 16;3(11):7865-7875. doi: 10.1021/acsabm.0c01022. Epub 2020 Oct 22. (IF= 4.0)
  15. Kumar, P., Shivam, P., Mandal, S., Prasanna, P., Kumar, S., Prasad SR, Kumar A, Das P, Ali V, Singh SK and Mandal, D. (2019). Synthesis, characterization and mechanistic studies of a gold nanoparticle – Amphotericin B covalent conjugate with enhanced antileishmanial efficacy and reduced cytotoxicity. International Journal of Nanomedicine.14,6073-6101.[ Corresponding author] (impact factor 7.0)
  16. Nayak S, Prasad SR, Mandal D, Das P. (2020). Carbon dot cross-linked polyvinylpyrrolidone hybrid hydrogel for simultaneous dye adsorption, photodegradation and bacterial elimination from waste water. Journal of Hazardous Materials Feb 12;392:122287. doi: 10.1016/j.jhazmat.2020.122287 (Imapct factor 14.3)
  17. Kumari, S., Prasad, SR., Mandal, D. and Das, Prolay (2019). Carbon Dot-DNA-Protoporphyrin Hybrid Hydrogel for Sustained Photoinduced Antimicrobial Activity. J Colloid Interface Sci. Jun 12; 553:228-238. (Impact factor 9.8)
  18. Mandal, S., Prasad, SR., Mandal, D. and Das, Prolay. (2019) BSA amplified ROS generation from Anthrarufin derived Carbon dot and concomitant Nanoassembly for combination antibiotic-photodynamic Therapy application. ACS Applied Materials and Interfaces. 11, 33273-33284 (Impact factor 10.3
  19. Nonsense suppression in Bhattacharya A, Köhrer C, Mandal D, Raj Bhandary U.L. (2015). PNAS 112(19), 6015-6020. (Impact Factor =9.8)
  20. Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine Mandal, D., Kohrer, C., Su, D., Ramesh Babu, I., Chan, C.L.T., Liu, Y., Soll, D., Blum, P., Kuwahara, M., Dedon, P.C. & RajBhandary, U.L. (2014). RNA 20, 177-188. (Impact factor = 4.3)
  21. Life Without tRNAIle-Lysidine synthetase; translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical-tRNA2Ile. Kohrer, , Mandal, D., Gaston, K.W., Grosjean, H., Limbach, P.A. & RajBhandary, U.L. (2014). Nucleic Acid Research. 42(3), 1904-1915. (Impact factor = 18.8)
  22. The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome. Voorhees, R., Mandal, D., Neubauer, , Köhrer, C., RajBhandary, U.L. and Ramakrishnan, V. (2013). Nature Structural & Mol. Biology. May; 20(5): 641–643. (Impact factor = 13.8)
  23. Agmatidine, a modified cytidine on the anticodon of archaeal tRNAIle, base pairs with adenosine but not with Mandal D, Kohrer C, Su D, Russel, SP, Krivos, K, Castleberry CM, Blum P, Limbach PA, Soll, D and Rajbhandary UL (2010) PNAS, 107(7), 2872-2877. (Impact factor = 9.8)
  24. Identification and characterization of a tRNA decoding the rare AUA codon in Haloarcula Köhrer C, Srinivasan G, Mandal D, Mallick B, Ghosh Z, Chakrabarti J and  Rajbhandary UL (2008). RNA, 14 (1), 117-126. (Impact factor = 4.3)Analysis of the topology of Vibrio cholerae NorM and identification of amino acid residues involved in Norfloxacin resistance. Singh, A. K., Haldar, R, Mandal, D. and Kundu, M. (2006). Agents Chemother. 50(11), 3717-3723. (Impact factor = 4.6)
  25. Fas-, caspase-8, and caspase 3-dependent signaling regulates the activity of aminophospholipid translocase and phosphatidylserine externalization in human erythrocytes. Mandal, D., Mazumder, A., Das, P., Kundu, M. and Basu, J. (2005) J. Biol. Chem. 280(47), 39460-39467. (Impact factor = 3.8).